FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686784
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1252.867073E-740.3233036
CTGTCCG1102.5361893E-432.7275739
TATTAGG2258.5128704E-1032.0049552
GCACGGG900.003750966432.0026256
GTCCAGG2051.2132659E-831.6192531
GACCGTG1153.2904136E-431.3069117
GTCTGGA3550.030.4272462
GTATTAG4100.029.8626271
ACCGCGA1255.3625536E-428.8023598
CGCCAGG1555.7515903E-527.8793431
GGGCGCA1555.76016E-527.8732537
GCCCTAC1306.736607E-427.700631
TAGACCG3900.027.6945765
GTGTAAG2105.27938E-727.4368111
GCTATAC2105.2894757E-727.4308173
GTACACT3453.6379788E-1227.130684
CTACTAG2405.615766E-827.0081121
TTAGGTA2405.631773E-827.0002464
GTGCGTG1607.15383E-527.0002469
AGACCGC2156.4965025E-726.7928946
Produced by FastQC (version 0.11.2)