FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700336
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG952.0137904E-645.4735767
CCTACAC2005.456968E-1239.5970733
TTATACG1502.761044E-838.3999064
TACACCG2250.038.3999065
CTGTGCG1604.874346E-835.9999129
TAACTCG800.002106573235.9999125
GACCCGC1251.3259498E-534.5599146
AGTGTGC1905.800757E-934.1051838
TCCGTTC1301.7343213E-533.2306868
CCCGCCT1301.7343213E-533.2306868
ACCGCGC1102.536413E-432.7271928
ACAGCGC1402.8784645E-530.8570678
GTCTACA6100.030.6950241
AGACCCG1652.5173686E-630.545385
TGGATCG1902.204888E-730.3157185
CCGTTCG950.004889104530.3157189
GGATCGG2401.7007551E-929.9999286
TAGGGAG1204.2247222E-429.9999285
GTCCTAG1453.6523732E-529.7994161
TAAGTAG1453.6577185E-529.793035
Produced by FastQC (version 0.11.2)