FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010741
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3800.036.0029951
TATACCG3600.033.997785
CTAGACA4050.033.7755744
TATACAG5300.032.6016435
ACTCCGC2456.002665E-1132.3276188
TAGACAG5100.029.6451245
GTCCGGG1255.3642824E-428.8023951
TCTAGAC4500.028.7981223
ACACAGT4550.028.4816556
GACCGCG1555.7685927E-527.8691487
ACCGCGC2105.2958603E-727.4294958
GCCTAAT2705.984475E-926.6688841
GTCTTAG3255.638867E-1126.5868261
TAATAGT3800.026.5245844
GGACTGC6750.025.5983286
TGTGGAC6750.025.5983283
ATTATAG5650.025.4850623
CCTTAGG3409.822543E-1125.4138771
GTATAGA6250.025.346111
GTGGACT6550.025.2807944
Produced by FastQC (version 0.11.2)