FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989688
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG2501.8189894E-1234.564481
GGACAGC2456.002665E-1132.324186
GGATCGG3200.031.4977136
GACAGCG3350.030.0888867
GGACTGC4400.026.1799166
TACACCG3109.313226E-1025.5478215
GATCGGT4250.025.4112017
GTATTAG5200.024.9263061
ACAGCGT4400.024.544918
CTAGCCT3251.6079866E-924.3699254
ACTGTGC4303.6379788E-1223.4413418
TATACGG3102.60261E-823.2252925
TCGGTCG3153.0839146E-822.8566369
AGGATCG5050.022.8113775
CAGTACT5800.022.345464
TCTATAC6450.022.3273433
GTGCGGG1952.7376367E-422.1567151
AGACCGC1952.740446E-422.1533587
ACAGCGC3254.2942702E-822.1533578
GTACTAG6250.021.8908351
Produced by FastQC (version 0.11.2)