FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873670
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC2054.285921E-728.10078
TAACCCG1050.00796830327.4316375
GTCCTAC1358.409748E-426.6696471
GTCTAGT3201.3424142E-924.7527661
GTGTAAG3551.6916601E-1024.3407461
GTATTAG3902.1827873E-1124.002681
CTAGGAC2101.6396656E-524.0013053
GTATAGA5150.023.7696441
TAGGCAG3652.382876E-1023.6738785
TCTAGAC3352.2828317E-923.643083
GGATCGG2802.5009285E-723.1454436
TAGGTAG2202.3488165E-522.911655
GTCTAGG3153.0784577E-822.8596971
CTAGTAC4750.022.7380813
GGGTAGG3503.787136E-922.63111
TACGAGA3254.2866304E-822.156324
GTTCAGG4609.094947E-1221.9154911
CAGCGCG1650.002705447421.820629
GTCCTAA3004.8619404E-721.6024131
AGGACAC3004.8619404E-721.6024135
Produced by FastQC (version 0.11.2)