FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_yp2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_yp2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781796
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG1450.054.6120537
TACACCG2200.042.5414475
ACCGCGC2005.456968E-1239.5937428
AGACCGC2850.037.891176
TATACCG2900.037.2378735
CTATAGT2053.1468517E-1035.1186454
TCTGGAC3900.035.0736243
AAGACCG3500.034.9681365
TAGGTAG1459.1856236E-734.755355
CCGCGCA2104.0745363E-1034.2802939
ACCCTGC3850.033.6570138
CCTTATA3000.033.5946852
GTGGACT5150.033.550244
TGGACGC1301.7356671E-533.227645
CGGTCCG900.003755695231.9949389
TGTGGAC5900.031.7258263
GACTGCT7050.031.654577
GGACTGC7100.031.4336596
GGACCCT4250.030.4912456
GTCATAG3800.030.3168131
Produced by FastQC (version 0.11.2)