FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_yp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_yp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004326
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA1105.529808E-639.2631382
TACACCG4100.035.1133775
ACTGTGC4150.031.2181828
GACCGTG2351.364242E-930.6277717
TCTGGAC3300.030.5383
GGATCGC1204.2320034E-429.9926766
ACCGTGC4200.029.1328418
GTCCTAC3800.028.4141141
GTATAAG5350.028.2547821
GACTGTG2303.7427526E-828.164237
CTGGGCG2054.304111E-728.0879049
CTAGGGC1804.969379E-627.9931663
GCGTCCC1804.9732316E-627.9903779
GTGTAAG3403.6379788E-1227.5226921
TAGACAG2904.292815E-1027.3036775
AGTGCGC3505.456968E-1226.7336678
ACAGTGC4350.026.4736588
CTGGACA3850.026.1754264
CTAGGAC1959.255107E-625.8398443
CACCGTG3358.185452E-1125.782187
Produced by FastQC (version 0.11.2)