FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462023
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCG1651.4606485E-939.2648779
TAGGTAG1459.174637E-734.7554325
TACACCG1751.0711665E-732.9112665
TATACTG2601.200533E-1030.4587465
CCTTATA1902.2017593E-730.3162882
AGGATCG3450.029.2147145
GTATTAG2553.245077E-928.235761
CTATAGG1555.7587033E-527.871431
CTGAGCG1306.7529746E-427.6867719
CCATATA3153.8198777E-1127.4290262
GTGTAAG2405.62286E-827.0004481
ATACAGT3204.5474735E-1126.9975246
CTATAGT1907.5444477E-626.5238824
GGATCGG2757.2686817E-926.1794196
TAGGACA1959.230062E-625.8437844
CGTCTAG1400.001039690925.714711
CGTACAC1400.001040347925.7119273
CATATAC3109.276846E-1025.5460453
ATAGGAT1701.0803866E-425.4094333
TATACAG3409.822543E-1125.4094335
Produced by FastQC (version 0.11.2)