FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_mat0a1_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_mat0a1_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26514028
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG690270.2603414313358951No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA407420.15366205391349816No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT335230.1264349573742624No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT309300.11665522869629616No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG274360.10347729888495252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG649100.029.2670976
TACAGGC424250.029.01487
GAGCGCA108750.028.9927449
TATACAG777450.028.4353925
TAGAGCG114350.028.0136157
ACAGGCA457300.026.6344458
TGCAGTC158750.026.0735057
CAGGCAA496000.024.2370229
TACAGGT308700.020.8004887
GTATATA962150.019.7536141
TATATAC1182100.019.5420363
TATACCG192850.018.5892375
ATTATAC542750.018.515693
TCTATAC278550.018.3747223
ATATACA1151450.018.3179154
CAGTCTC261950.017.6700219
TATACGG172050.017.405665
ACAGGTT367200.017.3102198
ATAGAGC222800.017.2535296
GTATAAT392800.016.7517471
Produced by FastQC (version 0.11.2)