FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_mah58_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_mah58_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28626859
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG1458570.5095110155116913No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA1064470.3718431002157799No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT700670.24475965036890704No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT607360.21216438729795678No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG546510.1909081258268677No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT530540.18532944882286945No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT461450.16119477166530913No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC459240.1604227693998842No Hit
ATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAA436020.15231150577854174No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT430960.1505439349807815No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC425500.14863663526620227No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA383500.13396509900020814No Hit
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC363960.12713934141360042No Hit
TATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGC352280.12305925704248588No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC350590.12246890236892562No Hit
AGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTGTCCTT346130.12091092494639387No Hit
CAAATATACTATCTGTTTCAGGGAAAACTCCCAGGTTCGGATGTTCAAAA344430.12031707704991317No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT339110.11845868245622057No Hit
ACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACACGCACTATG338750.11833292643108348No Hit
GTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCT317030.11074564624781223No Hit
GTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTG316770.1106548224518799No Hit
ATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTG315690.1102775543764686No Hit
ATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGC307920.10756332016725971No Hit
ATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCA292260.10209293307379619No Hit
GTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCGGAAAC291650.10187984647564723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC227800.038.899617
TAGAGCG219850.035.231147
GAGCGCA221300.034.5776759
ATACAGG1150600.032.289486
TACAGGC764050.032.1649327
TATACAG1243300.031.6652935
TACAGGT448350.027.631517
CAGGCAA882950.027.4668489
ATAGAGC303100.026.7188746
ACAGGTT490850.025.6936748
TATACCG261200.024.6661515
CTCTGTG343550.024.6200437
TAATGCA450550.024.4934924
ATTATAC638350.024.132773
TCTATAC310000.023.7323913
ATGCAGT399650.023.4701636
ACCGATG240700.022.8186198
GTATAAT418050.022.7827761
GTATATA1414550.022.754191
CAGTCTC412650.022.4512589
Produced by FastQC (version 0.11.2)