FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_mah4_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_mah4_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20417359
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG351810.17230925899867852No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA349160.17101134382757338No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT215570.10558172582457896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG471200.027.4074215
ATACAGG383250.027.254316
TGCAGTC118100.027.1852137
TACAGGC234450.026.2213767
ACAGGCA258700.023.958228
GAGCGCA70650.022.6197919
TAGAGCG79200.020.6323417
CAGGCAA303250.020.2249099
GTATATA694300.019.9187971
TACAGGT211700.019.7221817
CTCTGTG167800.018.8330387
CAGTCTC186800.018.6901459
GTATTAG180650.018.2918511
TCTATAC211800.018.0817263
ATTATAC387150.017.8875163
TATACCG146300.017.7136965
TATATAC823150.017.3944233
TATACTG236100.017.2267535
ATGCAGT215500.017.1698236
TAATGCA285400.017.0508424
Produced by FastQC (version 0.11.2)