FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_mah148_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_mah148_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32074263
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG671660.20940777345375014No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA416120.1297364182615825No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT357880.11157855754939718No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT343450.10707962331044053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC196550.029.1894257
ATACAGG696050.028.4095656
TACAGGC448450.027.5932037
TATACAG850650.027.5285645
GAGCGCA127250.025.795829
TAGAGCG133450.025.406377
ACAGGCA487350.025.0066938
CAGGCAA538150.022.4857129
TACAGGT346250.020.2908547
CAGTCTC308400.020.2837249
ATTATAC644650.019.430223
TCTATAC348500.019.2928563
GTATATA1187050.018.8063931
GCAGTCT319700.018.5084238
TATACCG242600.018.4268555
TATATAC1401950.018.1462333
ATGCAGT350950.017.8861796
CTCTGTG264250.017.8701887
GTATTAG301500.017.7885971
CTATAAT625000.017.4964731
Produced by FastQC (version 0.11.2)