FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_mah137_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_mah137_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997457
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG678270.21881472405946076No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA446430.1440214918275393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC427550.029.0267417
ATACAGG658800.028.2463676
TATACAG810650.026.9869825
ACAGGCA463000.026.7731888
TGCAGTC177550.024.5697027
GAGCGCA119050.024.3683249
TAGAGCG123650.024.1602767
CAGGCAA518850.023.3502929
GTATATA1118700.018.720371
TATATAC1365100.018.4413363
TATACCG235100.017.8820485
TACAGGT331900.017.698277
ATATACA1289350.017.2913464
ATTATAC612600.017.2860953
TCTATAC341950.017.0312753
CAGTCTC283650.016.9782669
TATACTG353650.016.4065915
CTCTGTG254700.015.9120957
TATAGGG332850.015.8962915
GCAGTCT288250.015.7832218
Produced by FastQC (version 0.11.2)