FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686784
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTG1854.483809E-935.0165987
GTGGGCA2353.6379788E-1133.6920939
ACTGCGT2206.730261E-1032.717538
TAGGAGT2456.002665E-1132.3169024
TATACTG5400.030.6575365
GGGGCCG950.00489610930.3067595
TTAGGAC2151.9390427E-830.1327533
CTCAGGG1204.2301504E-429.993252
TAGACTT2651.5097612E-1029.877895
ACTGGTC2502.6393536E-928.7914248
GGGTACA2054.2963075E-728.0912381
TAAGGTA2054.299036E-728.0891915
CAGTCGA1306.7602145E-427.6840619
GCTCTAT3153.8198777E-1127.42241
GTGTACG2105.303682E-727.42241
GTCTTAG2156.5139466E-726.784671
TGTACAG3255.638867E-1126.5766985
GTATTAG3006.366463E-1026.394061
TAGGACT2207.9665915E-726.1740234
GGCCTAT1658.838198E-526.174026
Produced by FastQC (version 0.11.2)