FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700336
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCG657.5945846E-444.301547
CTGTGCG1252.872148E-740.31449
GCCCTAA1157.493958E-637.561
TAGGACA2151.2732926E-1136.8294644
CCCTAAT1001.4499952E-435.9950031
TTAGGAC1854.480171E-935.019663
CTAAGCA2900.034.7513164
TGTCCCG1051.9316335E-434.2809565
GACTGAC1708.317511E-833.877657
CTACACA2353.6379788E-1133.695044
CTTAGGA2206.712071E-1032.722732
CCTACAC2206.712071E-1032.7203943
CTAAGTA1102.5395092E-432.7203944
TACACGG1551.5471669E-632.5116125
TACACCG2009.578798E-932.39555
TAGAGCA2900.032.2690774
GGACTGC4100.031.6053686
CCCCTAT1601.9823037E-631.4956261
CTATATA2101.540866E-830.852861
GCCTAAC1402.881148E-530.8528581
Produced by FastQC (version 0.11.2)