FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010741
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC1701.9626896E-938.1084568
GACCGCG2451.8189894E-1235.25687
CTGTGCG2104.0745363E-1034.2774479
ACCGCGC2653.6379788E-1232.595918
TCGGTCG2559.640644E-1131.0513329
CCGCGCA2601.200533E-1030.4541939
CTGCGCC2003.464902E-728.7930559
CGGGGCT1753.9911065E-628.7930554
AGACCGC3351.8189894E-1227.9335616
ACTCCGC2853.5106495E-1027.782778
CGTACCG1607.16932E-526.9934885
GTCTACA8550.026.9422021
TATACAG5100.026.817065
GACCGTG2156.5199856E-726.7842357
CTATAAA3505.456968E-1226.737731
GATCGGT3505.456968E-1226.7364087
CCTAATA3255.638867E-1126.5782052
GTATTAG2757.2977855E-926.1767981
CCGCGCG1658.838841E-526.1755059
GTACATA4200.025.7093561
Produced by FastQC (version 0.11.2)