FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989688
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG2850.035.3596842
GTGTAGG3400.033.8756941
GCGTCAG3050.033.0376784
TAGGTAG2452.1318556E-929.3775275
TGTACCG2752.3283064E-1028.7899765
GTATTAG4800.026.9946941
TATTAGG5400.026.660082
TTAGGTA4100.026.3322944
GCAGCGT4450.024.2648931
CAGCGTC4500.023.9940722
ATTAGGT5100.023.9916463
CAGTGCG1500.001559319423.9904379
CTGTGTG3301.9299478E-923.9904359
CTATGCA6400.023.6155839
CTGCACG1851.9237374E-423.3420439
CCCTTAG5300.023.08981
CCCACAC9700.022.2602883
TTAGGCA2603.412706E-622.1461354
TAAGGTG5200.022.1461355
TGGACCC3656.180926E-921.6910765
Produced by FastQC (version 0.11.2)