FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n01_d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n01_d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873670
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG1902.2097811E-730.3097061
CGGGGCA950.00489724530.3062369
GTATTAG4350.028.1322821
TCTAGGG2054.2998E-728.0903153
GTCCTGG2904.292815E-1027.3048631
AGTGCGC1856.151464E-627.2346578
GGCCCGC1607.171838E-526.991496
TCGGTCG1959.258412E-625.838019
GGATCGG2259.69836E-725.591936
TCTATAC5350.025.5634963
TATTAGG4600.025.0370182
CATAGAG4151.8189894E-1224.282893
ACCTAGT2101.6429076E-523.9951841
CCCTAGT1500.001557897823.9938092
GTATAGA4950.023.2680571
ACCGCTC1550.001886041323.2184878
ACTGCTC3753.3651304E-1023.032748
ACCATGC4750.022.7296778
AGACCGC1902.3029665E-422.7296776
GAGGGTG5100.022.5811167
Produced by FastQC (version 0.11.2)