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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-05-15, 21:05 based on data in: /beegfs/mk5636/logs/html/HT5YKBCX2/2

        General Statistics

        Showing 16/16 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HT5YKBCX2_l02_n01_Ogla_104_kd
        26.4%
        47%
        23.4
        HT5YKBCX2_l02_n01_Ogla_108_kd
        26.4%
        47%
        26.0
        HT5YKBCX2_l02_n01_Ogla_109_kd
        23.2%
        47%
        15.7
        HT5YKBCX2_l02_n01_Ogla_110_kd
        28.4%
        47%
        21.4
        HT5YKBCX2_l02_n01_Ogla_174_kd
        26.5%
        48%
        21.4
        HT5YKBCX2_l02_n01_Ogla_89_kd
        23.3%
        45%
        20.4
        HT5YKBCX2_l02_n01_Ogla_91_kd
        24.2%
        46%
        20.1
        HT5YKBCX2_l02_n01_undetermined
        90.1%
        45%
        23.7
        HT5YKBCX2_l02_n02_Ogla_104_kd
        26.4%
        47%
        23.4
        HT5YKBCX2_l02_n02_Ogla_108_kd
        26.4%
        47%
        26.0
        HT5YKBCX2_l02_n02_Ogla_109_kd
        22.3%
        47%
        15.7
        HT5YKBCX2_l02_n02_Ogla_110_kd
        27.5%
        47%
        21.4
        HT5YKBCX2_l02_n02_Ogla_174_kd
        26.1%
        48%
        21.4
        HT5YKBCX2_l02_n02_Ogla_89_kd
        22.5%
        45%
        20.4
        HT5YKBCX2_l02_n02_Ogla_91_kd
        23.8%
        46%
        20.1
        HT5YKBCX2_l02_n02_undetermined
        89.8%
        44%
        23.7

        Lane 2 Demultiplexing Report

        Lane 2 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 8/8 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        23,697,674
        13.8
        Ogla_89_kd
        20,387,386
        11.9
        Ogla_91_kd
        20,052,274
        11.7
        Ogla_104_kd
        23,377,813
        13.6
        Ogla_108_kd
        25,978,136
        15.1
        Ogla_109_kd
        15,675,459
        9.1
        Ogla_110_kd
        21,356,201
        12.4
        Ogla_174_kd
        21,374,575
        12.4

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CGAGGCTGTCTTTCCC
        105624.0
        0.5
        AAGAGGCATCTTTCCC
        96388.0
        0.4
        CAGAGAGGTCTTTCCC
        92786.0
        0.4
        GCTACGCTTCTTTCCC
        78843.0
        0.3
        CGAGGCAGTCTTTCCC
        45705.0
        0.2
        TTCCATACTCTTTCCC
        41477.0
        0.2
        CGAGGCTGTCNTTCCC
        37739.0
        0.2
        TTCTATACTCTTTCCC
        35736.0
        0.1
        AAGAGGCATCNTTCCC
        35337.0
        0.1
        CAGAGAGGTCNTTCCC
        33165.0
        0.1
        AAGAGGAATCTTTCCC
        32385.0
        0.1
        CAGAGAAGTCTTTCCC
        31434.0
        0.1
        TTCCATAATCTTTCCC
        29232.0
        0.1
        GCTACGATTCTTTCCC
        28964.0
        0.1
        AAGAGGCAATCCTCTT
        28696.0
        0.1
        ATCCATAATCTTTCCC
        27782.0
        0.1
        TCCCATACTCTTTCCC
        27464.0
        0.1
        GCTACGCTTCNTTCCC
        27113.0
        0.1
        CGAGGCTGATCCTCTT
        26096.0
        0.1
        GCCCATACTCTTTCCC
        24374.0
        0.1

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        2.0
        178,907,904
        171,899,518
        13.8
        12.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        16 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.