FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23697674
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1711150.7220750863565767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT19350.023.8082182
GTATAGT12450.016.4029981
GTATAGG11750.015.3592441
CCGTATC14700.015.35059548-49
TAGACTG8100.015.245615
ACTGCGC7550.015.0991728
TAGGACC8000.014.8424494
GTATATA28250.014.7940921
CCTAGTA11150.014.4823882
GTATTAT16800.013.8519491
TATACTG16300.013.6951485
CCTACAC15700.013.613373
AGCGGTT13100.013.59807710-11
ATAATAC15850.013.4845363
GTATAGA13750.013.1251721
GTCTTAG8000.013.060411
CTACACT13250.012.904534
GTGTAAG8850.012.8792941
GTAGGAC9600.012.8633743
GTCCTAG8600.012.7014551
Produced by FastQC (version 0.11.5)