FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_91_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_91_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20052274
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN545770.27217361980990284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG121200.016.9681035
GTATAGG86350.016.2796381
GTATAAG87400.016.1383951
GTATTGG182150.015.9304261
TACACCG74350.015.3312755
TACACTG87500.015.3069645
CCTAGAC68500.014.9763423
CTATACT118050.014.4435624
TATACAG110850.014.1392465
TATACCG61600.014.1097185
GTATTAG96850.014.0243181
GTATATA256850.013.8489751
GTGTATG132150.013.8359481
CCTATAC90000.013.7206113
GTCTAGG78600.013.7157221
TACACAG84250.013.6988585
TGCGTTC131150.013.65448959
TCTATAC91150.013.5996073
TAGACTG63000.013.4192265
GTGTAGG99650.013.2966511
Produced by FastQC (version 0.11.5)