FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_89_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_89_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20387386
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN552430.27096656726860424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG134600.016.0905695
GTATTGG177850.015.9687191
GTATAGG95400.015.880531
TACACCG75050.015.1251185
CTATACT131350.015.114644
GTCTAGG79850.014.5718231
CCTATAC96050.014.5377533
TATACCG66300.014.399065
TAGACTG65700.014.3136845
GTATAAG95600.014.2079311
TACACTG98250.013.8739775
ACTGTGC92800.013.8198658
TATACAG119400.013.7633265
TAGACAG74650.013.7428095
TATTGGG190400.013.6693582
GTATATA281450.013.6680491
CCTACAC90400.013.5549713
CCTAGAC68500.013.5204573
TACACAG90350.013.5100385
ACAGTGC105600.013.4041828
Produced by FastQC (version 0.11.5)