FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_174_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_174_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21374575
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN578630.2707094760948463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG119350.017.312025
GTATAGG91300.017.2708841
GTATTGG172250.015.7718991
GTATATA222550.015.5791041
GTATAAG81250.015.0230111
TACACTG109000.014.9032345
TATACAG108350.014.8172875
TACACCG87450.014.610785
TATACCG65200.014.4972755
CTATACT119500.014.1104664
TACACAG90750.013.8701185
TAATACT127650.013.84213354
GTATAGA101300.013.6905411
TAGACTG60550.013.6494765
GTGTAGG105650.013.6213571
TATTGGG183700.013.5224712
CCTAGAC80750.013.4701633
GTATTAT119900.013.4285081
CTACACT107050.013.3998894
GTCTAGG89650.013.2445431
Produced by FastQC (version 0.11.5)