FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_110_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_110_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21356201
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN576150.2697811282072125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG124400.018.0211965
GTATTGG179750.016.0641561
GTATAGG95250.016.0551411
CCTAGAC76700.015.6050133
TACACAG89300.015.5307855
TATACCG69250.015.0891555
GTATAGA110300.014.7686841
GTATATA242450.014.691371
TATACAG111250.014.6865725
CTATACT125600.014.294314
TACACTG102550.014.2651745
GTATAAG85800.014.17019751
TCTATAC97300.013.9120453
GTATTAT135950.013.6939921
TTATACT134600.013.5149584
TAGACTG67700.013.3299035
TACACCG81050.013.2439475
GTATTAA118200.013.2190931
GTCTATG88400.012.8938381
GTCTAGG91200.012.8624991
Produced by FastQC (version 0.11.5)