FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_109_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_109_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15675459
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN425220.2712647840168508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG88200.016.2633445
TATACCG54050.016.2572695
GTATTGG152850.016.0644551
GTATATA181600.015.90115451
GTATAAG63950.015.8190341
GTATAGG69050.015.544821
TAGACTG50650.014.6290685
TACACTG75650.014.2524165
ACTGTGC72400.014.2371588
GTCTAGG64250.014.0449891
TACACCG60800.013.98366455
CCTAGAC56450.013.9672693
GTGTAAG46150.013.7903091
TATTGGG164000.013.785312
CTATACT94050.013.6861724
GTATTAG74950.013.4973621
TGCGTTC112950.013.2896679
TAGACAG61950.013.1873855
GTATAGA76050.013.177231
TACACAG69400.013.1405425
Produced by FastQC (version 0.11.5)