FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_108_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_108_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25978136
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN711680.2739534507017748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG144000.017.0527425
GTATAGG110900.016.2307171
GTATTGG236850.015.7407821
GTATAAG105250.015.6129181
TAGACTG77850.015.374755
GTATATA296400.015.1099681
TACACTG120150.014.8638135
GTATAGA125400.014.69481851
TATACCG82650.014.1945055
CTATACT142800.014.1359294
CCTAGAC85600.013.8160863
TATACAG128900.013.7811315
GTATTAT155450.013.748391
TACACCG94350.013.5921455
CCTATAC107850.013.3879133
TATTGGG254900.013.3221862
TCTATAC113950.013.2547713
GTGTAAG76000.013.248061
TGCGTTC170150.013.2051719
TAATACT170350.013.1881114
Produced by FastQC (version 0.11.5)