FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n02_Ogla_104_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n02_Ogla_104_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23377813
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN639370.27349435980174874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG101750.016.9439541
TATACTG141350.016.3646775
TATACCG79000.015.6322165
TACACTG106300.015.3262225
GTATAAG99300.015.1140341
ACTGTGC106950.014.8345548
CTATACT136100.014.5878584
GTATATA284900.014.42003251
TATACAG121900.014.3389855
TACACCG83150.014.3379085
GTATTGG187400.014.26861
TACACAG97350.014.1005545
TCTATAC106400.013.8832223
CCTAGAC82600.013.8582533
CTACACT105100.013.8290414
TAGACAG83450.013.5464325
CCTATAC110450.013.50315953
GTATATG193100.013.3309031
TAGACTG76050.013.053335
GTCTAGG89050.013.0136311
Produced by FastQC (version 0.11.5)