FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23697674
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG8000.017.1979855
TACACTG10800.016.6928065
CTATACC10900.016.5402894
CTACACT11250.016.4474324
CCTATAC12150.015.6195943
ATAATAC15200.015.2946143
CTAGGCT9950.015.2585374
TATACTG15750.015.0611795
TCTACAC12350.014.9824793
TAATACT15700.014.505334
GTCCTAA8900.014.3965631
GTATATA28250.013.7746461
TAATACA16900.013.7561024
CTAAGAC7700.013.5555753
ACTGCGC5652.928573E-1013.432958
TAGTACT25800.013.4242234
CTAGTAC16350.013.3483053
AATACAG12850.013.2913445
CCTTATA13300.013.1992122
GTATAGG10800.013.1820371
Produced by FastQC (version 0.11.5)