FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_91_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_91_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20052274
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG124250.018.426485
GTATTGG180750.016.0852431
CCTATAC88700.015.2091093
TACACTG95300.015.1022425
CTATACT124350.015.0507964
GTATTAG93500.014.9379251
TATACAG113400.014.7023175
CCTAGAC73450.014.5512333
GTATAGG86200.014.4944931
TATTGGG193500.014.3367132
TAGACTG60550.014.1989475
TATACCG65200.014.0605215
TGCGTTC137200.013.9864359
GTGTAGG92700.013.9393881
TAGACAG71600.013.9315045
ACTGTGC94300.013.8012578
GTATAAG84200.013.4846711
GTATATA267600.013.3679071
TCTATAC99800.013.32712653
ATACTGT129200.013.1249436
Produced by FastQC (version 0.11.5)