FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_89_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_89_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20387386
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133250.016.3621085
GTATAAG94350.016.0117031
GTATAGG94750.015.7936921
GTATTGG176150.015.6421941
TATACCG64750.015.478775
TACACCG71500.015.2132925
ACTGTGC90250.014.999498
TACACTG100350.014.8629855
CCTATAC91900.014.7830663
GTGTAGG90850.014.5892051
TATACAG119150.014.0726025
TAGACAG72750.013.9072215
GTATATA291100.013.7900821
TTATACT159600.013.7506464
CTACACT99050.013.6200244
CTATACT135100.013.6072264
TACACAG95700.013.5501615
CCTAGAC72350.013.5251683
TAGACTG67900.013.5014765
CTACACC116150.013.4961144
Produced by FastQC (version 0.11.5)