FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_174_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_174_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21374575
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG121450.016.8957145
TATACCG63850.016.7383235
GTATAGG88000.015.9792831
GTATAAG79550.015.70593551
CTATACT116050.015.5542884
CCTAGAC82500.015.4885233
TACACAG92350.015.3274415
TACACCG87750.014.6152745
TTATACT121900.014.4571234
GTATTGG177950.014.4159751
TACACTG103300.014.20850855
TATACAG108750.014.107945
GTATATA218350.014.0331361
CCTATAC87450.013.9599863
ATTATAC116800.013.827633
TAGACGG39500.013.5885055
GTGTAGG102800.013.4477031
GTATTAT118350.013.4469811
ACTGTGC102450.013.1668688
TATACTA141750.013.1021945
Produced by FastQC (version 0.11.5)