FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_110_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_110_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21356201
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125350.018.3785485
GTATAAG89850.015.7029771
GTATAGG97800.015.6894341
TATACCG69350.015.4109665
TACACCG80000.015.0218815
TAGACTG66750.015.0149885
CTATACT126300.014.8937114
GTATTGG184900.014.8245841
TACACTG94750.014.4380025
GTATATA236150.014.1621221
CCTATAC95950.014.1589863
TTATACT136550.013.9496644
CTACACT93850.013.6659744
TACACAG92650.013.4835465
GTATAGA107250.013.465421
CTAGTAC148350.013.3203883
TATACAG116050.013.3024625
TAGTACT223350.013.2924534
GTGTAGG90250.013.15940951
ACTGTGC95350.013.1013078
Produced by FastQC (version 0.11.5)