FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_109_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_109_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15675459
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG93950.016.3810945
GTATTGG152000.015.9396741
TATACAG84700.014.636995
CCTAGAC52800.014.30460553
TACACCG61000.014.2500355
TATTGGG163950.014.1683672
GTATAGG71300.014.125351
CTATACT96900.014.1182664
GTATAGA77900.013.9043361
GTATAAG63650.013.882491
GTATATA186450.013.7334381
TACACAG66050.013.6639245
TACACTG75500.013.6523515
TAGACTG50150.013.6391165
GTGTAGG75300.013.3749991
CCTATAC72300.013.3373773
ATACTGT92850.013.3010556
GTATTAG69100.013.2000641
TATACCG53050.013.0726045
TAATACT104850.012.9118694
Produced by FastQC (version 0.11.5)