FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_108_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_108_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25978136
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG146650.017.3609145
GTATAGG109600.016.6013151
GTATTGG218500.015.6108761
TACACTG119050.015.5206585
CTATACT145850.015.0793574
GTATATA294400.014.6360451
TACACAG104100.014.6012195
TATACAG132800.014.5574815
TACACCG93250.014.313555
GTATAGA120400.014.2835551
TCTATAC113750.014.1982813
GTATTAG116300.014.0926821
TAGACTG79250.014.0850525
TAATACT172850.013.905594
TATACCG84150.013.716465
ACTGTGC114800.013.5295978
CTACACT120300.013.3462524
CCTAGAC88750.013.2201653
GTCTAGG97050.013.1677321
GTATAAG103750.013.0958041
Produced by FastQC (version 0.11.5)