FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l02_n01_Ogla_104_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l02_n01_Ogla_104_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23377813
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG78400.017.5702655
TATACTG145600.017.0296445
GTATAGG105850.015.932881
GTATTGG191050.015.3175921
GTCTAGG87750.015.15891
CTATACT135150.015.1487294
GTATAAG99100.014.9088291
ACTGTGC105650.014.7913558
GTATAGA114150.014.6079071
TAGACTG76650.014.5630455
CCTAGAC82450.014.115313
TATACAG123250.014.0284895
GTATATA275600.014.01419451
GTGTAGG108600.013.9983541
CCTATAC102500.013.9031243
TACACCG88900.013.7852415
TTATACT153850.013.6779574
TACACTG109350.013.6397775
TACACAG99000.013.626355
TAGACAG84750.013.50746255
Produced by FastQC (version 0.11.5)