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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-05-15, 20:05 based on data in: /beegfs/mk5636/logs/html/HT5YKBCX2/1

        General Statistics

        Showing 16/16 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HT5YKBCX2_l01_n01_Ogla_120_kd
        26.0%
        47%
        17.1
        HT5YKBCX2_l01_n01_Ogla_70_kd
        25.5%
        47%
        19.6
        HT5YKBCX2_l01_n01_Ogla_72_kd
        24.8%
        47%
        18.6
        HT5YKBCX2_l01_n01_Ogla_79_kd
        24.6%
        46%
        16.4
        HT5YKBCX2_l01_n01_Ogla_82_kd
        23.6%
        47%
        14.5
        HT5YKBCX2_l01_n01_Ogla_86_kd
        24.1%
        46%
        16.9
        HT5YKBCX2_l01_n01_Ogla_87_kd
        25.6%
        45%
        18.3
        HT5YKBCX2_l01_n01_undetermined
        90.5%
        45%
        27.2
        HT5YKBCX2_l01_n02_Ogla_120_kd
        25.1%
        47%
        17.1
        HT5YKBCX2_l01_n02_Ogla_70_kd
        24.7%
        47%
        19.6
        HT5YKBCX2_l01_n02_Ogla_72_kd
        24.2%
        47%
        18.6
        HT5YKBCX2_l01_n02_Ogla_79_kd
        23.7%
        46%
        16.4
        HT5YKBCX2_l01_n02_Ogla_82_kd
        22.9%
        47%
        14.5
        HT5YKBCX2_l01_n02_Ogla_86_kd
        23.2%
        46%
        16.9
        HT5YKBCX2_l01_n02_Ogla_87_kd
        24.6%
        45%
        18.3
        HT5YKBCX2_l01_n02_undetermined
        90.1%
        44%
        27.2

        Lane 1 Demultiplexing Report

        Lane 1 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 8/8 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        27,233,450
        18.3
        Ogla_70_kd
        19,572,599
        13.2
        Ogla_72_kd
        18,600,896
        12.5
        Ogla_79_kd
        16,364,642
        11.0
        Ogla_82_kd
        14,517,876
        9.8
        Ogla_86_kd
        16,859,353
        11.3
        Ogla_87_kd
        18,307,028
        12.3
        Ogla_120_kd
        17,149,138
        11.5

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CGAGGCAGTCTTTCCC
        151506.0
        0.6
        CAGAGAAGTCTTTCCC
        144343.0
        0.5
        AAGAGGAATCTTTCCC
        123693.0
        0.5
        GCCACGATAAGGAGTA
        107845.0
        0.4
        CGCGGCAGGTAAGGAG
        105807.0
        0.4
        GCTACGATTCTTTCCC
        100498.0
        0.4
        CGCGGCAGAAGGAGTA
        99293.0
        0.4
        CACAGAAGCTAAGCCT
        95054.0
        0.3
        AAGAGGCATCTTTCCC
        92098.0
        0.3
        CGAGGCTGTCTTTCCC
        86442.0
        0.3
        CAGAGAGGTCTTTCCC
        86356.0
        0.3
        AACAGGAAGTAAGGAG
        86278.0
        0.3
        CACAGAAGAAGGAGTA
        81249.0
        0.3
        AACAGGAACTAAGCCT
        79859.0
        0.3
        TTCCATACTCTTTCCC
        73200.0
        0.3
        GCTACGCTTCTTTCCC
        70438.0
        0.3
        TTCTATACTCTTTCCC
        52709.0
        0.2
        ATCCATAATCTTTCCC
        48763.0
        0.2
        TTCCATAATCTTTCCC
        47151.0
        0.2
        TCCCATACTCTTTCCC
        42869.0
        0.2

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        1.0
        153,283,904
        148,604,982
        18.3
        15.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        16 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.