FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27233450
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2202750.808839864211108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT25900.021.8196032
GTATATA43100.016.5276241
CCGTATC19150.015.63034548-49
GTATAGG16150.015.2906961
TATACTG21650.014.4774785
TACTAGG18950.014.2845082
CCTATAC14800.013.7977683
TCCCTAC17650.013.7222189
GTACTAG26550.013.5939451
CTAGTAC25000.013.4871573
GTCCTAT16850.013.2462941
TACACCG13400.013.1130535
GCCTTAC8350.013.0809051
CGCCGTA24200.013.05554646-47
CCTAGTA16500.012.9517432
ACTGTGC16500.012.9516478
GTATAAC13600.012.9199031
TACACAG15300.012.7262165
CTATACT18500.012.57854
ACCGTGC17400.012.5546628
Produced by FastQC (version 0.11.5)