FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_87_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_87_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18307028
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN638860.3489698054757987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG181200.016.3277321
TATACTG116200.016.0207165
GTATAGG84800.015.5684361
TACACCG63700.014.9105115
GTATATA241200.014.0380981
TATACCG64350.013.9481065
GTATAAG88000.013.6531821
CCTAGAC61850.013.5903173
TAATACT139900.013.57811454
TATACAG104800.013.41325
TGCGTTC125350.013.3356929
CTATACT120050.013.2519534
TATTGGG202000.013.2123412
TACACTG84050.013.1649795
TAGACTG59600.013.1473945
GTCTAGG69100.013.1265731
GTATTAG92200.013.0827441
ATAATAC130450.013.0691213
TTATACT137400.013.0647854
CCTATAC82650.012.7557543
Produced by FastQC (version 0.11.5)