FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_86_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_86_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16859353
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN589150.3494499462701801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG105600.018.618575
CCTAGAC60300.016.145123
GTATAGG79800.015.5918621
GTATTGG152800.015.5398281
TACACTG79850.015.2256195
TAGACTG59900.015.1431585
GTCTAGG68500.014.9748581
GTATAAG76350.014.9280041
CCTATAC79550.014.3276763
CTATACT109600.013.8224864
GTATAGA91550.013.7982131
TATACAG99250.013.732875
TACACCG60650.013.4681395
TTATACT123650.013.4424974
TATACCG57850.013.3811725
TATTGGG164350.013.2630232
GTATATA213250.013.1835451
TCTATAC86700.013.1460993
ACAGTGC90600.013.1043438
GTCCTAG67150.013.0128181
Produced by FastQC (version 0.11.5)