FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_82_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_82_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14517876
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN511600.3523931462150524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG88250.017.4895045
CCTAGAC53800.016.860093
GTGTAGG74300.016.4266361
GTATAGG62650.016.2217241
GTATTGG123050.016.055211
TACACTG70500.015.4260655
CTACACT71400.015.0985914
TATACCG45650.014.9806445
ACTGTGC70950.014.8596778
CCTAGTA78000.014.612482
GTATAAG61150.014.6004321
TACACCG57050.014.2347315
TATACAG83550.014.2102715
GTATAGA74300.014.1256281
GTCTAGG67200.014.0633281
GTATATA180100.014.0545761
CTAGTAC109250.014.0407353
CCTACAC71050.013.9698263
TGCGTTC86650.013.8116899
CCTATAC69650.013.7733343
Produced by FastQC (version 0.11.5)