FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_79_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_79_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16364642
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN569810.3481958236544374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG102150.017.2950555
GTATAGG67900.015.8069471
TACACTG74450.014.86307055
GTATTGG138950.014.8333931
TATACCG52800.014.4813595
CCTAGAC57700.014.403753
GTATAGA87150.014.168221
GTCTAGG60050.013.9981161
CTACACT74800.013.9680494
CCTATAC74350.013.8609213
TCTATAC82800.013.8229283
GTGTAAG47550.013.7828141
GTACTAG119400.013.7619861
TATACAG91400.013.7175035
GTATAAG75850.013.6492961
CTATACT101350.013.5890444
TAGACAG62600.013.5798845
GTGTAGG74850.013.5144121
GTATATA203550.013.36881351
TACACCG58650.013.3608255
Produced by FastQC (version 0.11.5)