FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_72_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_72_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18600896
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN653590.3513755466403339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG109250.018.6917635
GTATTGG158300.017.3095281
TAGACTG60900.016.6878265
GTATAAG78450.015.5571681
TACACTG90650.015.5069775
TATTGGG171150.015.3996172
CCTAGAC67000.015.168243
TATACCG60200.015.1463495
GTATAGG79100.015.069111
CTATACT110650.015.0215244
ACTGTGC90200.014.6889368
CCTATAC80300.014.6075573
CTACACT91550.014.472494
TGCGTTC114550.014.1368679
GTCTAGG73850.014.0826371
GTATTAG90350.014.0337411
GTATAGA92450.013.9204321
TCTATAC89250.013.7280133
TACACCG72450.013.6997035
CTTACAC55700.013.6414653
Produced by FastQC (version 0.11.5)