FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_70_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_70_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19572599
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN689830.3524468058636464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG76750.016.7689575
GTATTGG162650.016.2633741
GTATAGG90050.016.0851731
CCTAGAC70500.015.4935743
GTATAAG81600.015.4810721
TAGACTG60950.015.4278925
TATACTG114750.015.3545235
TATACCG60650.015.1126865
TACACTG91600.014.8799535
GTATATA232900.014.7019631
GTGTAGG90900.014.6808771
CTATACT114550.014.5521124
TACACAG88350.014.3522455
GTATAGA100450.014.1834161
ACTGTGC90150.013.8020648
TATACAG107600.013.7707465
GTGTATG125450.013.6282911
TATTGGG173550.013.4633232
TCTATAC88850.013.362743
GTCTAGG82800.013.3066041
Produced by FastQC (version 0.11.5)