FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n02_Ogla_120_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n02_Ogla_120_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17149138
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN602580.351376261594023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG96800.016.3865325
GTATAGG79300.016.0497631
GTATTGG127800.014.8268551
CCTATAC81200.014.563113
TATACCG58600.013.9394865
ACTGTGC77750.013.7434428
GTATATA200100.013.7179041
CTATACT104750.013.6466034
GTATAGA84500.013.319831
GTCTTAG52050.013.229831
TATACAG100500.013.1842085
GTATAAG74000.012.8994551
GTGTAGG81550.012.8116691
ATACTGT96150.012.7931226
CCTAGAC57950.012.7024973
TACACTG78750.012.66445
TAGACTG57350.012.5871115
TATTGGG139250.012.3117642
GTCCTAG67600.012.2941461
GTATTAA95150.012.228251
Produced by FastQC (version 0.11.5)