FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27233450
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG21450.016.817125
GTATAGG15000.016.1411461
CTATACT20600.016.128614
CTAGTAC28550.015.7936923
TACACCG12900.015.4534295
TACACTG20000.014.4765535
CCTATAC15900.014.32881453
CCTACAC18250.014.3042773
GTCCTGG14750.014.1617231
GTATAGA20600.014.0578231
TATTGGG29250.013.9555422
TAGTACT41050.013.7594144
TATACAG19000.013.7396115
GTATATA45650.013.7274091
GTACCGT10500.013.5611736
CCTAGTA22100.013.53076652
TCTATAC14750.013.5152283
GTGTTAG14150.013.42021
CTACACC21300.013.3701964
ACAGTGC19100.013.176798
Produced by FastQC (version 0.11.5)