FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_87_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_87_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18307028
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG184450.015.7100711
TATACTG118700.015.3261225
GTATAGG84850.015.1720551
GTATAAG84250.014.9981831
TATACCG65900.014.6317025
GTGTAGG74550.014.4008371
TATACAG103700.014.3367215
GTATATA249550.014.1054711
CCTATAC83850.014.105283
TACACTG82900.014.0949935
TAGACTG56900.013.4399565
CTATACT117650.013.3231674
TGCGTTC129700.013.2204289
TACACCG67100.013.1666325
GTATAGA100500.013.0930461
ACTGCGC42850.013.0800528
GTATTAG91400.013.0453131
GTGCGTT140800.012.8191518
TAATACT147550.012.8123664
TATTGGG201200.012.7720522
Produced by FastQC (version 0.11.5)