FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_86_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_86_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16859353
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG108200.017.2085555
GTATTGG156800.016.2685891
GTATAGG79800.015.4771911
TACACTG80200.015.1618165
CCTAGAC63000.015.1544833
TAGACGG31050.014.685725
CTATACT107800.014.6727364
TAGACTG53800.014.6558655
ACTGTGC79550.014.3301338
GTATATA219250.014.2996711
GTATAAG71800.014.2906181
TACACCG63300.013.957095
TAGACAG60400.013.8408065
CCTATAC78650.013.5884343
TATACCG59150.013.5711795
ATACTGT104900.013.53883656
TACACAG72650.013.5338195
TATTGGG169650.013.5233832
TATACAG99100.013.4684725
TTATACT117850.013.4617814
Produced by FastQC (version 0.11.5)