FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_82_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_82_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14517876
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG87950.017.0124155
GTATTGG127700.016.6283151
CCTAGAC55450.015.5905373
GTATAGG66150.015.5833751
CCTATAC68700.014.8652763
TAGACAG55500.014.8062265
TAGACTG47150.014.7082995
GTATAAG58800.014.7036691
GTATAGA75000.014.694621
TATTGGG138200.014.6762672
TACACCG57600.014.5962755
CTAGACA79100.014.5321594
TACACTG73650.014.5111235
GTGTAGG66350.014.3192651
TATACCG49350.014.1488615
TACACAG62800.013.9927595
TATACAG80950.013.9653355
ACTGTGC68250.013.9190518
GTCTAGG65350.013.6660791
CTAGTAC113800.013.6488713
Produced by FastQC (version 0.11.5)