FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_79_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_79_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16364642
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG99700.018.1041875
TAGACTG54450.015.702375
GTATTGG138800.015.6411161
TACACCG60450.015.165325
GTATAGG72250.015.0570071
TACACTG77700.015.03850655
TATACCG54150.014.9121785
CCTAGAC58350.014.5714493
GTGTAGG74300.014.1300751
GTATATA207050.014.1104521
TATACAG93350.013.992945
CTATACT106400.013.9284744
ACTGTGC77750.013.8066418
CCTATAC73200.013.6918443
GTATAAG71650.013.5918541
GTATAGA87400.013.588441
TTATACT117200.013.2934244
GTCTAGG63500.013.1667921
TATTGGG157000.012.8587022
ACTGTCC54900.012.7181818
Produced by FastQC (version 0.11.5)