FastQCFastQC Report
Wed 15 May 2019
HT5YKBCX2_l01_n01_Ogla_72_kd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5YKBCX2_l01_n01_Ogla_72_kd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18600896
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG163200.018.4251651
CCTAGAC68600.016.2023333
GTATAGG85650.015.8623131
TATACTG108300.015.5259955
GTCTAGG78100.015.3885361
TAGACTG59550.015.3145465
TATTGGG175350.015.1424642
CCTACAC84700.014.8610083
TACACTG91700.014.8143065
GTGTAGG86850.014.2757381
CTACACT91750.014.029724
TACACAG84050.013.9586645
TACACCG74500.013.8990415
GTATAGA92800.013.8723211
ACTGTGC89750.013.8657478
CTATACT113600.013.839984
TGCGTTC117600.013.77284059
GTATAAG74200.013.7645621
ACAGTGC100500.013.5641418
GTATATA230550.013.4753631
Produced by FastQC (version 0.11.5)